Analysis of Genetic Diversity in Indigenous Cine Capari Sheep under Conservation by Microsatellite Markers

作者：Cemal Ibrahim; Yilmaz Onur^*; Karaca Orhan; Binbas Pelin; Ata Nezih

来源：Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013, 19(3): 383-390.

DOI：10.9775/kvfd.2012.7857

• Genetic resources
• Sheep
• Cine Capari
• Diversity
• Microsatellites

In this study, 123 animals from 3 different flocks (ADU-CCKP conservation flock and two breeders' flocks) were genotyped with 10 microsatellite markers to investigate genetic diversity in the endangered native Cine Capari sheep breed. The number of alleles per microsatellite marker ranged from 7 (OarCP34) to 17 (OarFCB193), with an average of 11.5 alleles per locus. Total number of alleles for the investigated 10 microsatellite markers was found to be 104, 72 and 45 for ADU-CCKP, EA and MV flocks, respectively. The considerable difference in the allele numbers of the flocks indicates high genetic variability in the ADU-CCKP flock versus the other two breeders' flocks. The observed alleles have different size from other shows the existence of private alleles for flocks. According to the polymorphism information content (PIC) values, the highest and lowest polymorphism was detected to be 0.875 and 0.699 for OarFCB193 and OarFCB304 markers, respectively. The mean expected heterozygosity (He) and the mean observed heterozygosity (Ho) for the whole Cine Capari population under conservation were 0.727 and 0.789, respectively. The highest genetic similarity (0.8262) was observed between the ADU-CCKP conservation flock and Erdogan Akturk's flock. The results obtained in the present study will help to interpret the genetic structure of indigenous Cine Capari sheep and will be of benefit to the efforts for conservation of this breed.

• 出版日期2013-6