Arterivirus RNA-dependent RNA polymerase: Vital enzymatic activity remains elusive

作者：Lehmann Kathleen C; Gorbalenya Alexander E; Snijder Eric J; Posthuma Clara C^*

来源：Virology, 2016, 487: 68-74.

DOI：10.1016/j.virol.2015.10.002

• Equine arteritis virus
• Nidovirus
• In vitro RdRp activity
• Recombinant nsp9
• Polymerase active site mutant
• Reverse genetics

All RNA viruses encode an RNA-dependent RNA polymerase (RdRp), which in arteriviruses is expressed as the C-terminal domain of nonstructural protein 9 (nsp9). Previously, potent primer-dependent RdRp activity has been demonstrated for the homologous polymerase subunit (nsp12) of the distantly related coronaviruses. The only previous study focusing on the in vitro activity of nsp9 of an arterivirus (equine arteritis virus; EAV) reported weak de novo polymerase activity on homopolymeric RNA templates. However, this activity was not retained when Mn2+ ions were omitted from the assay or when biologically relevant templates were supplied, which prompted us to revisit the biochemical properties of this polymerase. Based on the properties of active-site mutants, we conclude that the RNA-synthesizing activities observed in de nova and primer-dependent polymerase and terminal transferase assays cannot be attributed to recombinant EAV nsp9-RdRp. Our results illustrate the potential pitfalls of characterizing polymerases using highly sensitive biochemical assays.

• 出版日期2016-1