Absence of occult hepatitis B virus infection in sera of diabetic children and adolescents following hepatitis B vaccination

作者：Elrashidy Heba; El Didamony Gamal; Elbahrawy Ashraf^*; Hashim Alaa; Alashker Ahmed; Morsy Mohamed Hanafy; Elwassief Ahmed; Elmestikawy Amr; Abdallah Abdallah Mahmoud; Mohammad Abdel Gawad Saeid; Mostafa Mohamed; George Nilly M; Abdelhafeez Hafez

来源：Human Vaccines & Immunotherapeutics, 2014, 10(8): 2336-2341.

DOI：10.4161/hv.29521

• Children
• Egypt
• HBV
• IDDM
• occult HBV infection
• vaccination

Background: The prevalence of occult hepatitis B (HB) infection (OBI) in HB-vaccinated diabetic children has not yet been tested. Here, we aimed to determine the prevalence of OBI among HB-vaccinated children and adolescents with insulin-dependent diabetes mellitus (IDDM).
Results: Eighty-seven (51.2%) children had a titer for antibodies to HB surface antigen (anti-HBs) of <10 IU/L. These included 44 (70%) IDDM children and 43 (40.2%) healthy children. Eighty-three (48.8%) children had an anti-HBs titer of >= 10 IU/L; they included 19 (30%) with IDDM and 64 (59.8%) healthy children. None of the enrolled children (n = 170) were reactive for total antibody to HB core antigen (anti-HBc) as determined by enzyme-linked immunosorbent assay. HB virus DNA was not detected in HB-vaccinated IDDM or healthy children and adolescents.
Method: An amount of 170 HBsAg-negative sera samples from HB-vaccinated children and adolescents was included. They were classified into the IDDM group (n = 63) and the healthy control group (n = 107). HBsAg, anti-HBc, and anti-HBs were tested by enzyme-linked immunosorbent assay, and HB virus DNA was tested by nested polymerase chain reaction using 3 pairs of surface, core, and X genes. In
Conclusion: Primary HB vaccination confers long-term protection against OBI in Egyptian diabetic children and adolescents. However, the number of cases tested in this study was relatively low, and further studies and long-term follow-up of large populations are needed to draw solid and convincing conclusions.

• 出版日期2014