Dynamics of pneumococcal carriage among day-care center attendees during the transition from the 7-valent to the higher-valent pneumococcal conjugate vaccines in Greece

作者：Grivea Ioanna N; Priftis Kostas N; Giotas Apostolos; Kotzia Doxa; Tsantouli Alexandra G; Douros Konstantinos; Michoula Aspasia N; Syrogiannopoulos George A^*

来源：Vaccine, 2014, 32(48): 6513-6520.

DOI：10.1016/j.vaccine.2014.09.016

• Streptococcus pneumoniae
• Nasopharyngeal carriage
• 7-Valent pneumococcal conjugate vaccine
• 10-Valent pneumococcal conjugate vaccine
• 13-Valent pneumococcal conjugate vaccine
• Day-care centers

Background: In Greece recently, higher-valent pneumococcal conjugate vaccines (PCVs) replaced the 7-valent (PCV7); the 10-valent (PCV10) became available in May 2009 and the 13-valent (PCV13) in June 2010. %26lt;br%26gt;Methods: We investigated the nasopharyngeal colonization with Streptococcus pneumoniae in day-care center attendees in Athens and the prefecture of Viotia. Between December 2010 and June 2011, nasopharyngeal cultures were obtained 4 times, at enrollment and then every 6 to 8 weeks. %26lt;br%26gt;Results: Among the 233 children, 225 (96.6%) had been vaccinated with %26gt;= 1 dose of PCV7. One tenth of the PCV7 vaccinated attendees had also received %26gt;= 1 dose of PCV13 or PCV10. During the 4 samplings, 358 isolates were recovered from a total of 874 samples. Of the 233 children, 183 (78.5%) were found to carry S. pneumoniae at least once. The overall serotype distribution among carriers was similar regardless of the time lapsed since the last PCV7 dose. A high frequency of 19A (17.1%) coincided with a low frequency of 19F (1.4%). Non-PCV13 serotypes accounted for 73.1% of the isolates; 23B, 15B/C, 16F, 21, 11A, 15A, 6C, 10A, 22F and 23A were the most common. Among attendees aged 24-59 months (median age 42 months), prolonged carriage of a non-PCV13 serotype was relatively common, mainly for 21 and 16F. One out of 4 cases of colonization with the prevalent non-PCV13 serotypes was followed by persistent carriage for 5 to 14 weeks. %26lt;br%26gt;Conclusions: During this period of transition to the higher-valent PCVs in the day-care center setting, non-PCV13 serotypes dominated and exhibited prolonged colonization. The frequency and the duration of prolonged carriage tends to be increased, if sampling frequency increases and the carriage time before and after positive cultures is taken into consideration. Further studies regarding the fitness of the colonizing non-PCV13 serotypes will likely to be seen in the future.

• 出版日期2014-11-12