Association Atudy for 26 Candidate loci in Idiopathic Pulmenary Fibrosis Patients from Four European Populations

作者：Kishore Amit; Zizkova Veronika; Kocourkova Lenka; Petrkova Jana; Bouros Evangelos; Nunes Hilario; Lostakova Vladimira; Mueller Quernheim Joachim; Zissel Gernot; Kolek Vitezslav; Bouros Demosthenes; Valeyre Dominique; Petrek Martin

来源：Frontiers in Immunology, 2016, 7: 274.

DOI：10.3389/fimmu.2016.00274

• MUC5B
• MUC2
• cytokines
• idiopathic pulmonary fibrosis
• sequenom MassARRAY
• single nucleotide polymorphism
• association study
• network analysis

Idiopathic pulmonary fibrosis (IPF) affects lung parenchyma with progressing fibrosis. In this study, we aimed to replicate MUC5B rs35705950 variants and determine new plausible candidate variants for IPF among four different European populations. We genotyped 26 IPF candidate loci in 165 IPF patients from four European countries, such as Czech Republic (n = 41), Germany (n = 33), Greece (n = 40), France (n = 51), and performed association study comparing observed variant distribution with that obtained in a genetically similar Czech healthy control population (n = 96) described in our earlier data report. A highly significant association for a promoter variant (rs35705950) of mucin encoding MUC5B gene was observed in all IPF populations, individually and combined [odds ratio (95% confidence interval); p-value as 5.23 (8.943.06); 1.80 X 10-11]. Another non-coding variant, rs7934606 in MUC2 was significant among German patients [2.85 (5.051.60); 4.03 X 10-4] and combined European IPF cases [2.18 (3.161.50); 3.73 X 10-5]. The network analysis for these variants indicated genegene and genephenotype interactions in IPF and lung biology. With replication of MUC5B rs35705950 previously reported in U.S. populations of European descent and indicating other plausible polymorphic variants relevant for IPF, we provide additional reference information for future extended functional and population studies aimed, ideally with inclusion of clinical parameters, at identification of IPF genetic markers.

• 出版日期2016-7-11