Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation

作者：Kere Michel; Siriboon Chawalit; Lo Neng Wen; Ngoc Tan Nguyen; Ju Jyh Cherng^*

来源：Journal of Reproduction and Development, 2013, 59(1): 78-84.

• Culture system
• Embryo development
• Handmade cloning
• Parthenote
• Vitamin C

In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 mu g/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were tested. None of these vitamin C additions affected nuclear maturation of oocytes, yet supplementation at 50 mu g/ml led to significantly increased intracellular glutathione (GSH) levels and reduced reactive oxygen species (ROS). When cultured in IVM- and/or IVC-supplemented media, the group supplemented with 50 mu g/ml of vitamin C showed improved cleavage rates, blastocyst rates and total cell numbers per blastocyst (P%26lt;0.05) compared with other groups (control, 25 mu g/ml and 100 mu g/ml). In contrast, supplementation with 50 mu g/ml vitamin C decreased (P%26lt;0.05) the apoptosis index as compared with the groups supplemented with 100 mu g/ml. In addition, even with a lower blastocyst rate to start with (37.6 vs. 50.3%, P%26lt;0.05), supplementation of HMC embryos with vitamin C ameliorated their blastocyst quality to the extent of PA embryos as indicated by their total cell numbers (61.2 vs. 59.1). Taken together, an optimized concentration of vitamin C supplementation in the medium not only improves blastocyst rates and total cell numbers but also reduces apoptotic indices, whereas overdosages compromise various aspects of the development of parthenotes and cloned porcine embryos.

• 出版日期2013-2