A thioacetamide-induced hepatic encephalopathy model in C57BL/6 mice A behavioral and neurochemical study

作者：de Miranda Aline Silva; Rodrigues David Henrique; Vieira Luciene Bruno; Lima Cristiano Xavier; Rachid Milene Alvarenga; Teixeira Vidigal Paula Vieira; Gomez Marcus Vinicius; dos Reis Helton Jose; Guatimosim Cristina; Teixeira Antonio Lucio^*

来源：Arquivos de Neuro-Psiquiatria, 2010, 68(4): 597-602.

DOI：10.1590/S0004-282X2010000400022

• hepatic encephalopathy
• thioacetamide
• behavioral changes
• SHIRPA
• glutamate

Objective: Hepatic encephalopathy (HE) is a neuropsychiatric syndrome resulting from liver failure. In the present study, we aimed to standardize an animal model of HE induced by thioacetamide (TAA) in C57BL/6 mice evaluating behavioral symptoms in association with liver damage and alterations in neurotransmitter release. Method: HE was induced by an intraperitoneal single dose of TAA (200 mg/kg, 600 mg/kg or 1,200 mg/kg). Behavioral symptoms were evaluated using the SHIRPA battery. Liver damage was confirmed by histopathological analysis. The glutamate release was measured using fluorimetric assay. Results: The neuropsychiatric state, motor behavior and reflex and sensory functions were significantly altered in the group receiving 600 mg/kg of TM. Biochemical analysis revealed an increase in the glutamate release in the cerebral cortex of HE mice. Conclusion: HE induced by 600mg/kg TAA injection in C57BL/6 mice seems to be a suitable model to investigate the pathogenesis and clinical disorders of HE.

• 出版日期2010-8