科研之友
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摘要
Phytophthora sojae is the most damaging fungal pathogen for soy plants. Rhamnolipids produced by Pseudomonas aeruginosa were investigated for the antimicrobial effects against both P. sojae mycelia and zoospores. Of particular importance is the effectiveness against zoospores because P. sojae spreads infection primarily via zoospores. Mycelial growth on agar plates was evaluated with three medium compositions and the rhamnolipid effect compared for each medium. Against zoospores, the effects were examined with the rhamnolipid mixture and its separated mono- and di-rhamnolipids. The zoospore study was done in small dose increments to identify the minimum concentrations that cause all zoospores to lyse or stop moving. Rhamnolipids at 100 and 1000 mgL(-1) inhibited the mycelial growth by up to approximate to 30%. Rhamnolipids killed the zoospores much more effectively. The rhamnolipid mixture and its mono- and di-rhamnolipid fractions were similarly effective; the mixture might be slightly more potent, requiring only 8-20 mgL(-1) (14-34 mu M) concentrations.
- 出版日期2016-1
