ALTERATION OF DNA-PROTEIN CROSSLINKS AND DNA DAMAGE IN MOUSE F9 EMBRYONIC CARCINOMA CELLS INDUCED BY FLUORIDE

作者：Peng, Wei; Xu, Shangrong; Zhang, Jun^*

来源：Fluoride, 2016, 49(2): 143-155.

• Cell viability
• DNA-protein crosslinks
• DNA damage
• Fluoride
• F9 embryonic carcinoma cells

The aim of this in vitro study was to evaluate the effects of the fluoride ion (F) on DNA-protein crosslinks (DPCs) and DNA damage in F9 embryonic carcinoma cells exposed to 0, 50, 100, and 150 mg F/L. After incubation for 24 hr, the proportions of viable cells were measured with the Cell Counting Kit-8 (CCK-8). The levels of lactate dehydrogenase (LDH) leakage and reactive oxygen species (ROS) were measured spectrophotometrically. Fluorometric spectrophotometry and single-cell gel electrophoresis (SCGE) were used to determine the levels of DPCs and DNA damage in the F9 cells, respectively. Cell viability decreased in the groups treated with 100 and 150 mg F/L compared with the control. LDH leakage levels, ROS levels, and DPCs levels increased with increasing concentrations of F compared with the control group. On SCGE, the tail length increased with increasing concentrations of F, and the effects were dose-dependent. In conclusion, excessive F in F9 cells reduced cell viability, increased LDH leakage and ROS levels, and induced DPCs and DNA damage.

• 出版日期2016-6
• 单位青海省畜牧兽医科学院