Modular Scanning Confocal Microscope with Digital Image Processing

作者:Ye Xianjun*; McCluskey Matthew D*
来源:PLos One, 2016, 11(11): e0166212.
DOI:10.1371/journal.pone.0166212

摘要

In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip carrier, and a piece of etched silicon wafer. The data collected by the CCD were processed to yield images of the specimen. By selecting effective pixels in the recorded CCD images, a virtual pinhole is created. By analyzing the image moments of the imaging data, a lateral resolution enhancement is achieved by using a 20 x / NA = 0.4 microscope objective at 532 nm laser wavelength.

  • 出版日期2016-11-9