MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex

作者：Takahashi Hidehisa; Takigawa Ichigaku; Watanabe Masashi; Anwar Delnur; Shibata Mio; Tomomori Sato Chieri; Sato Shigeo; Ranjan Amol; Seidel Chris W; Tsukiyama Tadasuke; Mizushima Wataru; Hayashi Masayasu; Ohkawa Yasuyuki; Conaway Joan W; Conaway Ronald C; Hatakeyama Shigetsugu^*

来源：Nature Communications, 2015, 6(1): 5941.

DOI：10.1038/ncomms6941

Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. Recently, the little elongation complex (LEC)-which contains the transcription elongation factor ELL/EAF-was found to be required for the transcription of Pol II-dependent small nuclear RNA (snRNA) genes. Here we show that the human Mediator subunit MED26 plays a role in the recruitment of LEC to a subset of snRNA genes through direct interaction of EAF and the N-terminal domain (NTD) of MED26. Loss of MED26 in cells decreases the occupancy of LEC at a subset of snRNA genes and results in a reduction in their transcription. Our results suggest that the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes.

• 出版日期2015-1
• 单位河北医科大学