Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells

作者：Perrier Emeline; Ronziere Marie Claire; Bareille Reine; Pinzano Astrid; Mallein Gerin Frederic; Freyria Anne Marie^*

来源：Biotechnology Letters, 2011, 33(10): 2091-2101.

DOI：10.1007/s10529-011-0653-1

• Bone morphogenetic protein-2, chondrogenesis
• Collagen
• Fibroblast growth-factor-2
• Mesenchymal stem cells
• Transforming growth factor-beta3

Adult mesenchymal stem cells (MSCs) are currently being investigated as an alternative to chondrocytes for repairing cartilage defects. As several collagen types participate in the formation of cartilage-specific extracellular matrix, we have investigated their gene expression levels during MSC chondrogenic induction. Bone marrow MSCs were cultured in pellet in the presence of BMP-2 and TGF-beta 3 for 24 days. After addition of FGF-2, at the fourth passage during MSC expansion, there was an enhancing effect on specific cartilage gene expression when compared to that without FGF-2 at day 12 in pellet culture. A switch in expression from the prechondrogenic type IIA form to the cartilage-specific type IIB form of the collagen type II gene was observed at day 24. A short-term addition of FGF-2 followed by a treatment with BMP-2/TGF-beta 3 appears sufficient to accelerate chondrogenesis with a particular effect on the main cartilage collagens.

• 出版日期2011-10