Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes

作者：Yang, Wucai; Tang, Keqiong^*; Wang, Yaning; Zhang, Yingying; Zan, Linsen^*

来源：Scientific Reports, 2017, 7(1): 15080.

DOI：10.1038/s41598-017-12780-y

• ADIPOSE TRIGLYCERIDE LIPASE
• HORMONE-SENSITIVE LIPASE
• LIPID DROPLET SIZE
• ADIPOCYTE DIFFERENTIATION
• GRANULOSA-CELLS
• IN-VITRO
• FAT
• ADIPOGENESIS
• EXPRESSION
• LIPOLYSIS

Melatonin (N-acetyl-5-methoxytryptamine) is a derivative of tryptophan which is produced and secreted mainly by the pineal gland and regulates a variety of important central and peripheral actions. To examine the potential effects of melatonin on the proliferation and differentiation of bovine intramuscular preadipocytes (BIPs), BIPs were incubated with different concentrations of melatonin. Melatonin supplementation at 1 mM significantly increased peroxisome proliferator-activated receptor gamma(PPAR gamma), CCAAT/enhancer-binding protein (C/EBP)beta, and C/EBP alpha expression and promoted the differentiation of BIPs into adipocytes with large lipid droplets and high cellular triacylglycerol (TAG) levels. Melatonin also significantly enhanced lipolysis and up-regulated the expression of lipolytic genes and proteins, including hormone sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL), and perilipin 1 (PLIN1). Moreover, melatonin reduced intracellular reactive oxygen species (ROS) levels by increasing the expression levels and activities of superoxide dismutase 1 (SOD1) and glutathione peroxidase 4 (GPX4). Finally, the positive effects of melatonin on adipogenesis, lipolysis, and redox status were reversed by treatment with luzindole, anantagonist of nonspecific melatonin receptors 1 (MT1) and 2 (MT2), and 4-phenyl-2-propionamidotetraline (4P-PDOT), a selective MT2 antagonist. These results reveal that melatonin promotes TAG accumulation via MT2 receptor during differentiation in BIPs.

• 出版日期2017-11-8
• 单位西北农林科技大学