Amino acid divergence between the CHS domain contributes to the different intracellular behaviour of Family II fungal chitin synthases in Saccharomyces cerevisiae

作者：Jimenez Cristina; Sacristan Carlos; Roncero M Isabel G; Roncero Cesar^*

来源：Fungal Genetics and Biology, 2010, 47(12): 1034-1043.

DOI：10.1016/j.fgb.2010.08.013

• Cell wall
• Chitin synthase
• Heterologous expression
• Antifungal

Family II chitin synthases (CS) including classes IV and V enzymes share conserved catalytic domains flanked by transmembrane regions Here we addressed the characterization of Family II fungal CSs by heterologous expression in Saccharomyces cerevisae Full-length CSs from classes V or IV were not functional when expressed in S cerevisiae and accumulated in different intracellular compartments However the exchange between different class IV but not of class V CHS domains resulted in functional proteins both in vivo and in vitro The different domains afford the chimeric proteins distinct intracellular behaviours ranging from endoplasmic reticulum retention to reduced endocytic turnover at the plasma membrane These results allow a role in chitin synthesis to be assigned to all class IV enzymes but they also highlig

• 出版日期2010-12