Melatonin decreases neuronal excitability in a sub-population of dorsal root ganglion neurons

作者：Oliveira Abreu Klausen; Ferreira da Silva Francisco Walber; da Silva Alves Kerly Shamyra; Silva dos Santos Nathalia Maria; Cardoso Teixeira Ana Carolina; do Amaral Fernanda Gaspar; Cipolla Neto Jose; Leal Cardoso Jose Henrique^*

来源：Brain Research, 2018, 1692: 1-8.

DOI：10.1016/j.brainres.2018.04.027

• Melatonin
• DRG
• Dorsal root ganglion
• Excitability
• MT1
• Action potential

Melatonin, a powerful antioxidant, participates in the regulation of important physiological and pathological processes. We investigated the actions of melatonin on neuronal excitability of intact dorsal root ganglions (DRG) from rats using intracellular recording techniques in current clamps. Melatonin blocked the generation of action potentials in a concentration-dependent manner. Bath applied melatonin (1.0-1000.0 nM) hyperpolarized the resting membrane potential, and increased the input resistance and rheobase. Melatonin also altered the active electrophysiological properties of the action potential, amplitude and maximum descendant inclination, in a statistically significant way. In order to provide evidence on the mechanism of action of melatonin in the DRG, quantitative PCR (qPCR) was performed. Analyses were performed for melatonin membrane receptors, MT1 and MT2, and it was observed that the DRG expresses MT1 receptors. In addition, we noted that the melatonin-induced effects were blocked in the presence of luzindole, a melatonin receptor antagonist. The minimal effective concentrations of melatonin (10.0 nM) and the blockade of effects caused by luzindole suggest that the effects of melatonin are hormonal, and are induced when it binds to MT1 receptors.

• 出版日期2018-8-1