Measuring piecemeal microautophagy of the nucleus in Saccharomyces cerevisiae

作者：Millen Jonathan I; Krick Roswitha; Prick Tania; Thumm Michael; Goldfarb David S^*

来源：Autophagy, 2009, 5(1): 75-81.

DOI：10.4161/auto.5.1.7181

• autophagy
• piecemeal microautophagy of the nucleus
• yeast
• nucleus-vacuole junctions
• nucleus
• vacuole

Piecemeal microautophagy of the nucleus (PMN) selectively removes and degrades small fragments of the Saccharomyces cerevisiae nucleus. Interorganelle contact sites called nucleus-vacuole (NV) junctions determine the selectivity of PMN by establishing a platform for the biogenesis of PMN blebs and vesicles. PMN structures can be observed by fluorescence microscopy using GFP-tagged reporters; however, this approach is best supported with quantitative immunoblot assays of PMN-specific cargo degradation. Together, these assays should facilitate the further study of this fascinating but poorly understood autophagic process in different genetic backgrounds, physiological states, and environmental conditions.

• 出版日期2009-1