Differential cell surface expression of rhesus macaque's major histocompatibility complex class I alleles Mamu-B*1703 and Mamu-B*0101

作者：Ouyang, Dongyun; He, Xianhui^*; Xu, Lihui; Wang, Xichao; Gao, Qi; Guo, He

来源：Acta Biochimica et Biophysica Sinica, 2010, 42(4): 281-287.

DOI：10.1093/abbs/gmq019

• major histocompatibility complex class I
• rhesus macaque
• Mamu-B*17
• Mamu-B*01

The major histocompatibility complex class I allele Mamu-B*17 of rhesus macaques is an elite controller of simian immunodeficiency virus (SIV) infection whereas Mamu-B*01 has no inhibitory effect on SIV replication. The mechanism is still elusive. In this study, the so-called 'missing G' in the leading peptide sequence of Mamu-B*1703 allele was artificially inserted back through PCR amplification, and the new sequence was renamed as Mamu-B*1703(+G). The plasmids harboring Mamu-B*1703, Mamu-B*1703(+G) and Mamu-B*0101 cDNA sequence fused to EGFP gene were transfected into K562 and Cos-7 cells, respectively. Our data showed that these plasmids had similar transfection efficiencies and expression potentials in K562 cells, but the surface density of Mamu-B*1703 complexes, which was slightly influenced by the artificial change of the leading peptide length, was much higher than that of Mamu-B*0101 molecules. These results might partially account for the differential effects of Mamu-B*17 and Mamu-B*01 alleles on SIV replication in rhesus macaques.

• 出版日期2010-4
• 单位华南师范大学; 暨南大学