A high-throughput screening assay for identification of inhibitors of the A(1)A(O)-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1

作者：Aung Htin Lin; Dey Debjit; Janssen Peter H; Ronimus Ron S; Cook Gregory M^*

来源：Journal of Microbiological Methods, 2015, 110: 15-17.

DOI：10.1016/j.mimet.2014.12.022

• A(1)A(O)-ATP synthase
• Methanobrevibacter ruminantium
• Methane
• High-throughput screening
• Inhibitor

We report the development of a high-throughput screening platform to identify inhibitors of the membrane-bound A(1)A(O)-ATP synthase from the rumen methanogen Methanobrevibacter ruminantium Ml. Inhibitors identified in the screen were tested against growing cultures of M. ruminantium, validating the approach to identify new inhibitors of methanogens.

• 出版日期2015-3