Neuroprotective effect of Tinospora cordifolia ethanol extract on 6-hydroxy dopamine induced Parkinsonism

作者：Kosaraju Jayasankar^*; Chinni Santhivardhan; Roy Partha Deb; Kannan Elango; Antony A Shanish; Kumar M N Satish

来源：INDIAN JOURNAL OF PHARMACOLOGY, 2014, 46(2): 176-180.

DOI：10.4103/0253-7613.129312

• Complex I activity
• dopamine
• iron asymmetry
• levodopa
• neurodegeneration

Objective: The present study investigates the neuroprotective activity of ethanol extract of Tinospora cordifolia aerial parts against 6-hydroxy dopamine (6-OHDA) lesion rat model of Parkinson's disease (PD).
Materials and Methods: T. cordifolia ethanol extract (TCEE) was standardized with high performance thin layer chromatography using berberine. Experimental PD was induced by intracerebral injection of 6-OHDA (8 mu g). Animals were divided into five groups: sham operated, negative control, positive control (levodopa 6 mg/kg) and two experimental groups (n = 6/group). Experimental groups received 200 and 400 mg/kg of TCEE once daily for 30 days by oral gavage. Biochemical parameters including dopamine level, oxidative stress, complex I activity and brain iron asymmetry ratio and locomotor activity including skeletal muscle co-ordination and degree of catatonia were assessed.
Results: TCEE exhibited significant neuroprotection by increasing the dopamine levels (1.96 +/- 0.20 and 2.45 +/- 0.40 ng/mg of protein) and complex I activity (77.14 +/- 0.89 and 78.50 +/- 0.96 nmol/min/mg of protein) at 200 and 400 mg/kg respectively when compared with negative control group. Iron asymmetry ratio was also significantly attenuated by TCEE at 200 (1.57 +/- 0.18) and 400 mg/kg (1.11 +/- 0.15) when compared with negative control group. Neuroprotection by TCEE was further supported by reduced oxidative stress and restored locomotor activity in treatment groups.
Conclusion: Results show that TCEE possess significant neuroprotection in 6-OHDA induced PD by protecting dopaminergic neurons and reducing the iron accumulation.

• 出版日期2014-4