A mechanistic study of helicases with magnetic traps

作者：Hodeib Samar; Raj Saurabh; Manosas Maria; Zhang Weiting; Bagchi Debjani; Ducos Bertrand; Fiorini Francesca; Kanaan Joanne; Le Hir Herve; Allemand Jean Francois; Bensimon David; Croquette Vincent^*

来源：Protein Science, 2017, 26(7): 1314-1336.

DOI：10.1002/pro.3187

• magnetic traps
• helicases
• polymerase
• primase
• fork regression
• DNA unwinding
• Holliday junction migration
• ds-DNA fork
• active unwinding
• helicase
• polymerase coupling
• replisome
• primosome
• strand annealing

Helicases are a broad family of enzymes that separate nucleic acid double strand structures (DNA/DNA, DNA/RNA, or RNA/RNA) and thus are essential to DNA replication and the maintenance of nucleic acid integrity. We review the picture that has emerged from single molecule studies of the mechanisms of DNA and RNA helicases and their interactions with other proteins. Many features have been uncovered by these studies that were obscured by bulk studies, such as DNA strands switching, mechanical (rather than biochemical) coupling between helicases and polymerases, helicase-induced re-hybridization and stalled fork rescue.

• 出版日期2017-7