Differentiation of lactic acid bacteria based on RFLP analysis of the tuf gene

作者：Park Seok Hwee; Jung Jong Hyun; Seo Dong Ho; Lee Hye Lim; Kim Gye Won; Park Sun Young; Shin Woo Chang; Hong Sungyoul; Park Cheon Seok^*

来源：Food Science and Biotechnology, 2012, 21(3): 911-915.

DOI：10.1007/s10068-012-0119-9

• Lactobacillus
• tuf gene
• restriction fragment length polymorphism (RFLP)
• lactic acid bacteria

The tuf gene, which encodes the elongation factor Tu, is universally distributed in Gram-positive bacteria. A small region of the tuf gene was evaluated for the differentiation of Lactobacillus strains using restriction fragment length polymorphism (RFLP) analysis. The 803 bp portion of tuf genes from 9 closely-related Lactobacillus reference strains were successfully amplified by polymerase chain reaction (PCR), and restriction patterns of each were evaluated with a set of restriction enzymes, which included AluI, HaeIII, and Sau3AI. The results implied that restriction patterns obtained from 2 of these restriction enzymes (AluI and HaeIII) could effectively differentiate closely-related Lactobacillus species.

• 出版日期2012-6