Mulberry 1-Deoxynojirimycin Inhibits Adipogenesis by Repression of the ERK/PPAR gamma Signaling Pathway in Porcine Intramuscular Adipocytes

作者：Wang, Guo-qiang; Zhu, Li; Ma, Mei-lin; Chen, Xiao-chang; Gao, Yun; Yu, Tai-yong; Yang, Gong-she; Pang, Wei-jun^*

来源：Journal of Agricultural and Food Chemistry, 2015, 63(27): 6212-6220.

DOI：10.1021/acs.jafc.5b01680

• DNJ
• pork
• intramuscular adipocyte
• adipogenesis
• ERK/PPAR gamma signaling pathway

Intramuscular fat (IMF); which is Modulated by adipogenensis of intramuscular adipocytes, plays a key role in pork quality associated with marbling, juiceness, and flavor. However, the regulatory mechanism of 1-deoxynojirimycin (DNJ) on adipogenesis is still unknown. Here, we found that both DNJ (2.0, 3:0, 4.0, 5.0, and 6.0 mu M) and rosiglitazone (RSG; 0.1, 0.2, 0.3, 04, and 0.5 mM) had no effect on cell viability. Moreover, 41 mu M DNJ significantly inhibited adipogenesis, whereas 0.4 mM RSG increased lipogenesis of porcine intramuscular adipocytes. Interestingly, DNJ Sharply inhibited phosphorylation of extracellular regulated protein kinases 1/2 (ERK1/2), but did not change phosphorylation of AKT (protein kinase B) in intramuscular adipocytes. We further found that the inhibitory adipogenesis Of DNJ was attenuated by RSG via up-regulation of PPAR gamma. On the basis of the above findings, we suggest that DNJ inhibited adipogenesis through the ERK/PPAR gamma signaling pathway in porcine intramuscular adipocytes.

• 出版日期2015-7-15
• 单位西北农林科技大学