科研之友
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摘要
In mammals, retinoid-related orphan receptors (ROR) consist of three members as ROR alpha, ROR beta and ROR gamma. It is well known that ROR alpha plays a critical role in cerebellum development while ROR gamma t directs T helper 17 (Th17) cell differentiation. So far, the knowledge on fish ROR family is limited as only zebrafish ROR family members have been characterized, showing that they play roles in embryonic and cerebellar development. In this study, we have cloned two paralogues for ROR alpha (ROR alpha 1 and ROR alpha 2) and ROR gamma (ROR gamma 1 and ROR gamma 2) from grass carp (Ctenopharyngodon idellus). Phylogenetic analysis showed that grass carp ROR alpha and ROR gamma were grouped in the clade of zebrafish ROR alpha and ROR gamma, respectively. Real-time RT-PCR assay revealed that these four ROR transcripts exhibited similar expression patterns, in particular the high levels in pituitary, brain and some immune-related tissues, suggesting that all of them may play a role in endocrine and immune system of teleost. To explore the immune roles of grass carp ROR alpha and ROR gamma, their expression was detected in periphery blood lymphocytes (PBLs) challenged by immune stimuli. Results showed that both ROR alpha and ROR gamma mRNA levels were up-regulated by PHA but not LPS in PBLs, suggesting that their expression may be subject to different immune processes. In the same cell model, poly I:C stimulation induced ROR gamma 1/2 but not ROR alpha 1/2 expression, pointing to the different roles of grass carp ROR alpha and ROR gamma in immune response. Consistently, bacterial challenge significantly up-regulated the expression of these four ROR genes in spleen, headkidney and thymus. These results not only contribute to elucidate the roles of ROR in fish immunity but also facilitate to further clarify the existence of Th17-like cells in fish.
- 出版日期2012-8
- 单位电子科技大学
