High-throughput bacterial genome sequencing: an embarrassment of choice, a world of opportunity

作者：Loman Nicholas J; Constantinidou Chrystala; Chan Jacqueline Z M; Halachev Mihail; Sergeant Martin; Penn Charles W; Robinson Esther R; Pallen Mark J^*

来源：Nature Reviews Microbiology, 2012, 10(9): 599-606.

DOI：10.1038/nrmicro2850

Here, we take a snapshot of the high-throughput sequencing platforms, together with the relevant analytical tools, that are available to microbiologists in 2012, and evaluate the strengths and weaknesses of these platforms in obtaining bacterial genome sequences. We also scan the horizon of future possibilities, speculating on how the availability of sequencing that is %26apos;too cheap to metre%26apos; might change the face of microbiology forever.

• 出版日期2012-9