Anodic voltammetric methods for determination of the antiparasitic drug nitazoxanide in bulk form, pharmaceutical formulation, and its metabolite tizoxanide in human serum

作者：Radi Abd Elgawad^*; Abd El Ghany Nadia; Wahdan Tarek

来源：Monatshefte fur Chemie, 2012, 143(5): 697-702.

DOI：10.1007/s00706-011-0636-x

• Voltammetry
• Nitazoxanide
• Tizoxanide

The oxidative behavior of nitazoxanide was studied on a carbon paste electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetry. The oxidation process was shown to be irreversible over the pH range 3.4-11.0 and was adsorption-controlled process. An analytical method was developed for the determination of nitazoxanide in phosphate buffer solution at pH 5.6 as the supporting electrolyte. The anodic peak current varied linearly with nitazoxanide concentration in the range 4.0 x 10(-7) to 1.0 x 10(-5) M with a limit of detection of 7.5 x 10(-8) M. Validation parameters such as sensitivity, accuracy, precision, and recovery were evaluated. The proposed method was applied to the determination of nitazoxanide in the pharmaceutical formulation. The results were compared with those obtained by a spectrophotometric method. No difference was found statistically. Based on the adsorption behavior of the metabolite tizoxanide onto the carbon paste electrode surface, a simple and sensitive differential-pulse adsorptive anodic stripping voltammetric method was developed for the determination of tizoxanide in spiked human serum without the need for extraction.

• 出版日期2012-5