NMR chemical shift assignments for androcam, a testis-specific myosin VI light chain in D-melanogaster

作者：Joshi Mehul K; Moran Sean; MacKenzie Kevin R^*

来源：Biomolecular NMR Assignments, 2013, 7(2): 167-169.

DOI：10.1007/s12104-012-9402-1

• Calcium
• Calcium signaling
• Calmodulin-like
• Myosin VI

Androcam is a D. melanogaster calmodulin-like protein expressed exclusively in the testis that interacts with myosin VI and is critical to spermatogenesis. At micromolar free Ca2+, androcam binds two calcium ions using its C-terminal lobe but its N-terminal lobe is Ca2+-free. We are pursuing structural studies on androcam at physiological (10 mu M) and high (10 mM) calcium. Here we report the H-1, N-15, and C-13 chemical shifts of androcam at 10 mu M free Ca2+ determined using multi-dimensional NMR experiments.

• 出版日期2013-10