Neolignan inhibitors of antigen-induced degranulation in RBL-2H3 cells from the needles of Pinus thunbergii

作者：Hong Seong Su; Jeong Wonsik; Kim Jin Kyu; Kwon Jin Gwan; Lee Jae Yeon; Ahn Eun Kyung; Oh Junho; Seo Dong Wan; Oh Joa Sub

来源：Fitoterapia, 2014, 99: 347-351.

DOI：10.1016/j.fitote2014.10.015

• Pinus thunbergii
• Neolignan glycoside
• Pinusthunbergiside A
• beta-hexosaminidase

The ethanolic extract of the needles of Pinus thunbergii was found to suppress antigen mediated degranulation of rat basophilic leukemia (RBL-2H3) cells. A new neolignan glycoside, named pinusthunbergiside A (1), as well as six known neolignan glycosides (2-7) were isolated from the ethanolic extract using bioassay-guided fractionation. Their structures were elucidated by a combination of 1D and 2D NMR, HRESI-MS, and circular dichroism (CD) data. Compounds 2-7 were found for the first time in this plant. The inhibitory effects of isolated constituents on the release of (beta-hexosaminidase from RBL-2H3 cells were examined, and compounds 2, 3, 5, and 6 were found to show the inhibitory activity with IC50 values ranging between 52.3 and 75.3 mu M.

• 出版日期2014-12