Alkali extraction of rice residue protein isolates: Effects of alkali treatment conditions on lysinoalanine formation and structural characterization of lysinoalanine-containing protein

作者：Zhang, Zhaoli; Wang, Yang; Dai, Chunhua; He, Ronghai^*; Ma, Haile

来源：Food Chemistry, 2018, 261: 176-183.

DOI：10.1016/j.foodchem.2018.04.027

• Rice residue protein isolates (RRPI)
• Lysinoalanine-containing protein
• Alkali treatment
• Structural characterization

The influence of alkali extraction conditions on the formation of lysinoalanine (LAL) and the structural characterization of lysinoalanine-containing protein in rice residue protein isolates (RRPI) were explored in this study. It was found that LAL content increased from 0.256 to 13.079 g/kg as NaOH concentration increased from 0.03 to 0.09M and then decreased to 1.541 g/kg at 0.13M NaOH. The extraction temperature and time were found to have a positive correlation with LAL content. The highest LAL content (25.679 g/kg) was observed with alkali extraction using 0.09M NaOH at 75 degrees C for 120 min. The comparative structural analysis results showed that alkali treatment could degrade cystine, lysine, threonine and arginine to generate LAL; increasing alkali content would cause variations in secondary structure and micropore appearance on the surface of lysinoalanine-containing protein, whereas increasing alkali treatment temperature and time could enlarge the surface particle size of the protein.

• 出版日期2018-9-30
• 单位江苏大学