Downstream separation of poly(hydroxyalkanoates) using crude enzyme consortia produced via solid state fermentation integrated in a biorefinery concept

作者：Kachrimanidou Vasiliki; Kopsahelis Nikolaos^*; Vlysidis Anestis; Papanikolaou Seraphim; Kookos Ioannis K; Monje Martinez Belen; Escrig Rondan Maria Consuelo; Koutinas Apostolis A

来源：Food and Bioproducts Processing, 2016, 100: 323-334.

DOI：10.1016/j.fbp.2016.08.002

• Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
• Cell disruption
• Downstream processing
• Integrated processing
• Solid state fermentation
• Sunflower meal

Crude enzyme produced via solid state fermentation using the fungal strain Aspergillus oryzae was used to lyse cells of Cupriavidus necator, enabling the recovery of poly(3hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-12 mol% 3HV). A central composite design was employed in order to optimize the temperature and the pH value leading to the highest lysis of C. necator cells (88.9 +/- 0.4%) and the optimal hydrolysis of protein contained in bacterial cells. Enzymatic lysis of bacterial cells was also carried out at the optimum temperature and uncontrolled pH value leading to P(3HB-co-12 mol% 3HV) recovery yield and purity of 98% and 96.7%, respectively. The bacterial cell lysate obtained after the separation of P(3HB-co-12 mol% 3HV) granules was evaluated as nutrient-rich supplement together with crude glycerol for the production of poly(3-hydroxybutyrate) in shake flask cultures. This novel downstream separation process could be integrated in a sunflower-based biorefinery reducing the cost of poly(hydroxyalkanoate) recovery and fermentation medium formulation.

• 出版日期2016-10