Assessing the validity and reproducibility of genome-scale predictions

作者：Sugden Lauren A; Tackett Michael R; Savva Yiannis A; Thompson William A; Lawrence Charles E^*

来源：Bioinformatics, 2013, 29(22): 2844-2851.

DOI：10.1093/bioinformatics/btt508

Motivation: Validation and reproducibility of results is a central and pressing issue in genomics. Several recent embarrassing incidents involving the irreproducibility of high-profile studies have illustrated the importance of this issue and the need for rigorous methods for the assessment of reproducibility.
Results: Here, we describe an existing statistical model that is very well suited to this problem. We explain its utility for assessing the reproducibility of validation experiments, and apply it to a genomescale study of adenosine deaminase acting on RNA (ADAR)-mediated RNA editing in Drosophila. We also introduce a statistical method for planning validation experiments that will obtain the tightest reproducibility confidence limits, which, for a fixed total number of experiments, returns the optimal number of replicates for the study.

• 出版日期2013-11-15