Mitogen-activated protein kinases MpkA and MpkB independently affect micafungin sensitivity in Aspergillus nidulans

作者：Yoshimi Akira; Fujioka Tomonori; Mizutani Osamu; Marui Junichiro; Hagiwara Daisuke; Abe Keietsu^*

来源：Bioscience Biotechnology and Biochemistry, 2015, 79(5): 836-844.

DOI：10.1080/09168451.2014.998619

• micafungin
• mpkB
• cell wall signaling
• mpkA
• Aspergillus nidulans

The transcriptional regulation of the MAPK mpkA and cell wall-related genes in Aspergillus nidulans differs from that of their counterparts in Saccharomyces cerevisiae. The A. nidulans MAPK MpkB is putatively orthologous to the yeast MAPKs Kss1p and Fus3p. To investigate MpkB and its contribution to cell wall integrity in A. nidulans, we constructed mpkB-disruptant (mpkB increment ) strains. We previously showed that mpkA increment strains exhibited reduced colony growth and increased sensitivity to the beta-1,3-glucan synthase inhibitor micafungin. Like mpkA increment strains, mpkB increment strains exhibited slight growth retardation and increased sensitivity to micafungin. Although MpkB-dependent signaling modulated the transcription of some cell wall-related genes, the sugar composition of cell wall fractions was similar among wild-type, mpkA increment , and mpkB increment strains. To elucidate the relationship between MpkA and MpkB pathways, we compared conditional mutants of mpkB with those with mpkA deletion. Sensitivity testing suggested that MpkA and MpkB additively contribute to micafungin activity in A. nidulans.

• 出版日期2015-5-4