Analysis of illegally manufactured formulations of tadalafil (Cialis (R)) by H-1 NMR, 2D DOSY H-1 NMR and Raman spectroscopy

作者：Trefi Saleh; Routaboul Corinne; Hamieh Saleh; Gilard Veronique; Malet Martino Myriam^*; Martino Robert

来源：Journal of Pharmaceutical and Biomedical Analysis, 2008, 47(1): 103-113.

DOI：10.1016/j.jpba.2007.12.033

• tadalafil
• vardenafil
• homosildenafil
• Raman spectroscopy
• H-1 NMR
• 2D DOSY H-1 NMR
• LC-MS
• illegally manufactured drugs

Counterfeit and/or imitation medicines are becoming a major health problem not only in developing countries but also in wealthier countries. The need of new and easy analytical methods for quality control of drugs is essential. We describe the use of Raman spectroscopy, H-1 nuclear magnetic resonance (NMR) and 2D diffusion-ordered spectroscopy (DOSY) NMR to analyse genuine Cialis (R) and seven illegally manufactured formulations of this drug purchased via the internet. Seven out of the eight commercial formulations of tadalafil contain the active ingredient, measured by high performance liquid chromatography (HPLC), within 100 +/- 5% of stated concentration. Vardenafil and homosildenafil instead of tadalafil were found in the Chinese imitation. 2D DOSY NMR spectra clearly showed similarities and differences in the composition of the pharmaceutical formulations of tadalafil, thus giving a precise and global "signature" of the manufacturer. Our data show that the quality of the Cialis imitations manufactured in India and Syria is correct, whereas the Chinese formulation is adulterated with active pharmaceutical ingredients.

• 出版日期2008-5-12