An antibody-sensitized microfabricated cantilever for the growth detection of Aspergillus niger spores

作者：Nugaeva Natalia^*; Gfeller Karin Y; Backmann Natalia; Dueggelin Marcel; Lang Hans Peter; Guentherodt Hans Joachim; Hegner Martin

来源：Microscopy and Microanalysis, 2007, 13(1): 13-17.

DOI：10.1017/S1431927607070067

• biosensor
• microfabricated cantilever array
• fast fungal growth detection
• anti-Aspergillus niger polyclonal antibodies

We demonstrate a new sensitive biosensor for detection of vital fungal spores of Aspergillus niger. The biosensor is based on silicon microfabricated caritilever arrays operated in dynamic mode. The change in resonance frequency of the sensor is a function of mass binding to the cantilever surface. For specific A. niger spore immobilization on the cantilever, each cantilever was individually coated with anti-Aspergillus niger polyclonal antibodies. We demonstrate the detection of single A. niger spores and their subsequent growth on the functionalized cantilever surface by online measurements of resonance frequency shifts. The new biosensor operating in humid air allows quantitative and qualitative detection of A. niger spores as well as detection of vital, functional spores in situ within similar to 4 h. The detection limit of the sensor is 10(3) CFU mL(-1). Mass sensitivity of the cantilever sensor is similar to 53 pg Hz(-1).

• 出版日期2007-2