Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF

作者：Zhao L; Wei X; Ma Z; Feng D; Tu P; Johnstone B H; March K L; Du Y^*

来源：Neuroscience Letters, 2009, 452(3): 238-240.

DOI：10.1016/j.neulet.2009.01.025

• Excytotoxicity
• Brain-derived neurotrophic factor
• Adipose stromal cells
• Neuroprotection

The delivery of factors secreted by adipose stromal cells (ASC) to the brain may be a viable neuroprotective therapeutic option. In this study, we investigated the bioactivity of ASC-conditioned medium (ASC-CM) in glutamate-induced neurotoxicity and found that the ASC-CM significantly blocked glutamate neurotoxicity. We identified the brain-derived neurotrophic factor (BDNF) in the ASC-CM by using Western blot and demonstrated that this activity was critical for the neuroprotective effect of ASC-CM in excytotoxicity models. Furthermore, inactivating BDNF also attenuated the suppression by ASC-CM of glutamate-induced caspase-3 activity, but not p38 phosphorylation. These findings suggest that among ASC secrete a potent combination of factors, BDNF play a major role in neuroprotection against excytotoxicity.

• 出版日期2009-3-20
• 单位北京大学