High clonal heterogeneity of Panton-Valentine leukocidin-positive meticillin-resistant Staphylococcus aureus strains from skin and soft-tissue infections in the Province of Bolzano, Northern Italy

作者：Aschbacher Richard^*; Pichon Bruno; Spoladore Greta; Pagani Elisabetta; Innocenti Patrizia; Moroder Ludwig; Ganner Mark; Hill Robert; Pike Rachel; Ganthaler Oswald; Pagani Leonardo; Larcher Clara; Kearns Angela

来源：International Journal of Antimicrobial Agents, 2012, 39(6): 522-525.

DOI：10.1016/j.ijantimicag.2012.02.004

• Community-associated meticillin-resistant
• Staphylococcus aureus
• CA-MRSA
• SCCmec
• Arginine catabolic mobile element
• ACME
• Panton-Valentine leukocidin
• PVL
• ST93

Panton-Valentine leukocidin (PVL)-positive community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) isolates are widespread in many countries, with varying distribution and epidemiology. The aim of this study was to characterise 10 PVL-positive MRSA isolates collected during February 2010 to January 2011 from skin and soft-tissue infections in the North Italian Province of Bolzano. Accessory gene regulator (agr) typing, staphylococcal cassette chromosome mec (SCCmec) typing, staphylococcal protein A (spa) gene typing, multilocus sequence typing, toxin gene profiling, polymerase chain reaction for type I arginine catabolic mobile element (ACME) and antimicrobial resistance typing were applied to the isolates. Eight different CA-MRSA clones were identified, including ST30-IVc, ST772-V, ST80-IVc, ST5-IVc, ST88-IVa, ST93-IVa, ST8-IVc and the type I ACME-positive ST8-IVa. The high heterogeneity of PVL-positive MRSA probably reflects the introduction of different clones by international travellers or immigrants.

• 出版日期2012-6