MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation

作者：Zhang, Jiao Jiao; Wang, Xian Zhong; Do, Huynh Luong; Chandimali, Nisansala; Kang, Tae Yoon; Kim, Nameun; Ghosh, Mrinmoy; Lee, Sang Baek; Mok, Young Sun; Kim, Seong Bong; Kwon, Taeho^*; Jeong, Dong Kee^*

来源：Scientific Reports, 2018, 8(1): 8761.

DOI：10.1038/s41598-018-27123-8

• ATMOSPHERIC-PRESSURE PLASMA
• SIGNALING PATHWAY
• GENE-EXPRESSION
• HUMAN KERATINOCYTES
• OXIDATIVE STRESS
• GERM-CELLS
• AUTOPHAGY
• PROLIFERATION
• GROWTH
• MTOR

Non-thermal plasma treatment is an emerging innovative technique with a wide range of biological applications. This study was conducted to investigate the effect of a non-thermal dielectric barrier discharge plasma technique on immature chicken Sertoli cell (SC) viability and the regulatory role of microRNA (miR)-7450. Results showed that plasma treatment increased SC apoptosis in a time-and dose-dependent manner. Plasma-induced SC apoptosis possibly resulted from the excess production of reactive oxygen species via the suppression of antioxidant defense systems and decreased cellular energy metabolism through the inhibition of adenosine triphosphate (ATP) release and respiratory enzyme activity in the mitochondria. In addition, plasma treatment downregulated miR-7450 expression and activated adenosine monophosphate-activated protein kinase a (AMPKa), which further inhibited mammalian target of rapamycin (mTOR) phosphorylation in SCs. A single-stranded synthetic miR-7450 antagomir disrupted mitochondrial membrane potential and decreased ATP level and mTOR phosphorylation by targeting the activation of AMPKa, which resulted in significant increases in SC lethality. A double-stranded synthetic miR-7450 agomir produced opposite effects on these parameters and ameliorated plasma-mediated apoptotic effects on SCs. Our findings suggest that miR-7450 is involved in the regulation of plasma-induced SC apoptosis through the activation of AMPKa and the further inhibition of mTOR signaling pathway.

• 出版日期2018-6-8
• 单位西南大学