Adipose-Derived Mesenchymal Stem Cells Enhance Healing of Mandibular Defects in the Ramus of Swine

作者：Wilson Shanna M; Goldwasser Michael S; Clark Sherrie G; Monaco Elisa; Bionaz Massimo; Hurley Walter L; Rodriguez Zas Sandra; Feng Liang; Dymon Zaneta; Wheeler Matthew B^*

来源：Journal of Oral and Maxillofacial Surgery, 2012, 70(3): E193-E203.

DOI：10.1016/j.joms.2011.10.029

Purpose: This study investigated the effect of adipose-derived mesenchymal stem cells (ASCs) injected locally or systemically on the bone regeneration of a 10-mm-diameter cylindrical noncritical-size defect in the ramus of the pig mandible.
Materials and Methods: Fifteen Yorkshire pigs, weighing 60 to 80 kg, received bilateral 10-mm-diameter cylindrical surgical defects in each ramus of the mandible. Pigs received 1) a direct injection into the defect of 2.5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); 2) an ear vein injection of 5 million carboxy-fluorescein diacetate succinimidyl ester-labeled ASCs from 1 of 2 pig donors (n = 6); or 3) an ear vein injection of culture Dulbecco's Modified Eagle's Medium without stem cells (control; n = 3). Pigs from each treatment were sacrificed at 1 hour, 2 weeks, or 4 weeks after surgery. Healing of the defect was evaluated by dual-energy x-ray absorptiometry, micro-computed tomography, fluorescent microscopy, and histology.
Results: Bone healing was accelerated in the ASC-injected treatment groups at 2 and 4 weeks after surgery compared with the control pigs.
Conclusions: Results from this animal model provide evidence that the injection of ASC locally into a bone defect or systemically can accelerate the healing of bone.

• 出版日期2012-3