An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida

作者：Cheng Xi Yu; Tong Yen Wah; Loh Kai Chee^*

来源：Biochemical Engineering Journal, 2014, 87: 1-7.

DOI：10.1016/j.bej.2014.03.011

• Pharmaceuticals biotransformation
• Substrate inhibition
• Immersed hollow fiber membrane
• bioreactor
• Pseudomonas putida

An immersed hollow fiber membrane bioreactor (IHEMB) was fabricated for production of pharmaceutical intermediates cis-indandiol from indene by Pseudomonas putida ATCC 55687. At 2-6 g/L indene, significant increase of 160-400%, corresponding to 240-400 mg/L of cis-indandiol was achieved as compared to that obtained from suspension culture bioreactors (SCB). The IHFMB volumetric productivity of 10.1-12.9 mg/(Lh) and molar yield of 0.050-0.093 mol/mol indene was also higher than the 4.3-8.3 mg/(L h) and 0.01-0.030 mol/mol indene, respectively, in the SCB. The hollow fiber membranes in the IHFMB facilitated a more effective biotransformation through reactant sequestration and cell immobilization, with equal contribution at low indene concentrations, albeit a lower contribution by the latter at 6 g/L indene. The performance of the IHEMB was further improved by process optimization and the highest product titer of 487 mg/L, overall volumetric productivity of 24.6 mg/(Lh) and molar yield of 0.126 mol/mol indene were achieved with a module comprising 28 membranes (168 cm(2)/150 mL working volume) at a medium circulation rate of 5 mL/min and aeration rate of 0.5 vvm. Reusability of the membranes has also been successfully demonstrated through 5 successive operations of batch biotransformation.

• 出版日期2014-6-15