A composite polynomial approach for analyzing the indefinite self-association of macromolecules studied by sedimentation equilibrium

作者：Hall Damien^*

来源：Biophysical Chemistry, 2017, 228: 10-16.

DOI：10.1016/j.bpc.2017.06.002

A different approach is described for analyzing sedimentation equilibrium experiments of indefinitely self-associating systems. The procedure involves application of conservation of mass criteria, along with local evaluation of the weight average molar mass, to generate a polynomial based on a composite pseudo-independent variable. The outlined method does not depend upon non-linear regression to generate a solution, but instead requires evaluation of the roots of a high-order polynomial.

• 出版日期2017-9