Adsorption of calf thymus DNA on Au(110) studied by reflection anisotropy spectroscopy

作者：Cuquerella M Consuelo; Smith Caroline I; Fernig David G; Edwards Clive; Weightman Peter^*

来源：Langmuir, 2007, 23(4): 2078-2082.

DOI：10.1021/la062839j

Reflection anisotropy spectroscopy (RAS) was used to investigate the adsorption of single-stranded (ss-) and double-stranded (ds-) calf thymus DNA on Au(110) in an electrochemical cell. Both types of DNA form ordered structures for electrode potentials in the range from +0.6 to -0.4 V. Both types of DNA desorb at -0.6 V and may start desorbing at lower negative potentials. When adsorbed at +0.6 V, both forms give rise to a similar RAS signal and adsorb through the phosphate groups. As the potential is reduced, the RAS intensity observed from ss-DNA increases to roughly twice that observed from ds-DNA, a result that is interpreted as due to a change in the adsorption of the ss-DNA from sites involving the phosphate groups to sites involving the bases.

• 出版日期2007-2-13