A new Polyphenol oxidase biosensor mediated by azure B in laponite clay matrix

作者：Shan, D; Mousty, C^*; Cosnier, S; Mu, SL

来源：Electroanalysis, 2003, 15(19): 1506-1512.

DOI：10.1002/elan.200302740

• polyphenol oxidase
• tyrosinase
• catechol
• clay modified electrode
• azure B
• enzyme

Amperometric biosensor based on the entrapment of polyphenol oxidase within a laponite clay coating and crosslinked by glutaraldehyde is described for catechol detection. Laponite provides a hydrophilic enzyme surrounding increasing the long term stability of the biosensor compared to the corresponding biosensors obtained by chemical cross-linking of PPO with glutaraldehyde. Azure B, a cationic dye exchanged within the clay matrix, is used as an electron shuttle allowing the mediated detection of phenol derivatives at -0.05 V. The detection limits obtained with the optimized biosensor configuration for catechol, p-cresol and phenol are 1, 1 and 17 nM, respectively.

• 出版日期2003-10
• 单位扬州大学