Advanced glycation end products stimulate gene expression of fibroblast growth factor 23

作者：Baer Ludmilla; Waechter Kristin; Wege Nicole; Santos Alexander Navarrete; Simm Andreas; Foeller Michael

来源：Molecular Nutrition & Food Research, 2017, 61(8): 1601019.

DOI：10.1002/mnfr.201601019

• Calcium
• Diabetes
• Inflammation
• Phosphate
• Vitamin D

Scope: Osteoblasts produce fibroblast growth factor 23 (FGF23), a hormone inhibiting renal phosphate reabsorption and the formation of biologically active vitamin D, calcitriol. FGF23-deficient mice age rapidly and develop age-associated diseases at least in part due to massive calcification. Elevated FGF23 serum levels are detected in patients suffering from acute and chronic renal, cardiovascular, inflammatory, and metabolic diseases. Advanced glycation end products (AGEs) are sugar-modified proteins, nucleic acid, and lipids which contribute to these disorders. Here, we studied the significance of AGEs for the generation of FGF23.
Methods and results: As AGE sources, bread crust extract (BCE) and ribose-modified bovine serum albumin (r-BSA) were used. UMR106 osteoblast-like cells were exposed to BCE and r-BSA, and Fgf23 transcripts were determined by qRT-PCR. UMR106 cells express the receptor for AGEs, RAGE. BCE and r-BSA were powerful stimulators of Fgf23 transcription. NF kappa B inhibitor wogonin and store-operated calcium entry (SOCE) antagonist 2-APB attenuated the r-BSA and BCE effects on FGF23 synthesis.
Conclusion: Sources of AGEs induce the transcription of Fgf23 in UMR cells. At least in part, the effect is mediated through up-regulation of NF kappa B and subsequent SOCE. AGE-induced FGF23 productionmay contribute to increased FGF23 serum levels observed in chronic disease.

• 出版日期2017-8