A novel water-soluble beta-(1 -> 6)-D-glucan isolated from the fruit bodies of Bulgaria inquinans (Fries)

作者：Bi Hongtao; Ni Xiuzhen; Liu Xiaoyu; Iteku Jeff; Tai Guihua; Zhou Yifa^*; Zhao Jimin

来源：Carbohydrate Research, 2009, 344(10): 1254-1258.

DOI：10.1016/j.carres.2009.04.009

• Polysaccharide
• beta-(1 -> 6)-D-Glucan
• Bulgaria inquinans (Fries)
• Methylation

A low molecular-weight polysaccharide named BiWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (M(w)/M(n)) Was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear beta-(1 -> 6)-D-glucan without side chains. This is the first time to report a linear beta-(1 -> 6)-D-glucan with low molecular weight in non-lichenized ascomycete.

• 出版日期2009-7-6
• 单位东北师范大学; 沈阳农业大学; 长春师范大学