Application of thermal lens response to monitor health status of red blood cells: A quantitative study of the cell death process by extracting thermal diffusivity and size

作者：Vasudevan Srivathsan; Chen George Chung Kit^*; Andika Marta

来源：Applied Physics Letters, 2010, 96(11): 113703.

DOI：10.1063/1.3366723

• biological techniques
• biomedical measurement
• biothermics
• blood
• cellular biophysics
• photothermal effects
• thermal diffusivity
• thermal lensing

We explore monitoring the death process of individual red blood cells (RBC) quantitatively by using thermal lens (TL) response. TL response is a noninvasive excitation/probe technique that reflects photothermal parameters (e.g., absorption, thermal diffusivity, size, etc.). Since these parameters of cells change significantly during certain biological processes, real-time TL response was performed to monitor RBC death process when incubated with ionomycin. Theoretical model developed was applied to curve-fit the TL response for extracting thermal diffusivity and size of cells. Thermal diffusivity of dying RBC is found increased by 1.7 times in comparison with healthy cell.

• 出版日期2010-3-15
• 单位南阳理工学院