Azithromycin treatment is able to control the infection by two genotypes of Toxoplasma gondii in human trophoblast BeWo cells

作者：Ribeiro Mayara; Franco Priscila Silva; Lopes Maria Janice Buiate; Angeloni Mariana Bodini; Barbosa Bellisa de Freitas; Gomes Angelica de Oliveira; Castro Andressa Silva; da Silva Rafaela Jose; de Oliveira Fernanda Chaves; Balga Milian Iliana Claudia; Martins Filho Olindo Assis; Letta Francesca; Mineo Jose Roberto; Vieira Ferro Eloisa Amalia

来源：Experimental Parasitology, 2017, 181: 111-118.

DOI：10.1016/j.exppara.2017.08.004

• Trophoblast cell
• Azithromycin
• Toxoplasma gondii atypical strains
• Cytokines

Trophoblast infection by Toxoplasma gondii plays a pivotal role in the vertical transmission of toxoplasmosis. Here, we investigate whether the antibiotic therapy with azithromycin, spiramycin and sulfadiazine/pyrimethamine are effective to control trophoblast infection by two Brazilian T. gondii genotypes, TgChBrUD1 or TgChBrUD2. Two antibiotic protocols were evaluated, as follow: i) pretreatment of T. gondii-tachyzoites with selected antibiotics prior trophoblast infection and ii) post-treatment of infected trophoblasts. The infection index/replication and the impact of the antibiotic therapy on the cytokine milieu were characterized. It was observed that TgChBrUD2 infection induced lower infection index/replication as compared to TgChBrUD1. Regardless the therapeutic protocol, azithromycin was more effective to control the trophoblast infection with both genotypes when compared to conventional antibiotics. Azithromycin induced higher IL-12 production in TgChBrUD1-infected cells that may synergize the anti-parasitic effect. In contrast, the effectiveness of azithromycin to control the TgChBrUD2-infection was not associated with the IL-12 production. BeWo-trophoblasts display distinct susceptibility to T gondii genotypes and the azithromycin treatment showed to be more effective than conventional antibiotics to control the T gondii infection/replication regardless the parasite genotype.

• 出版日期2017-10