Genetic Identification of Cinnamomum Species Based on Partial Internal Transcribed Spacer 2 of Ribosomal DNA

作者：Lee Shih Chieh^*; Lee Chang Hui; Lin Min Yi; Ho Kuen Yih

来源：Journal of Food and Drug Analysis, 2010, 18(4): 225-231.

• Cinnamomum spp
• molecular identification
• internal transcribed spacer2 (ITS2)
• phylogenesis

The present study investigates the genetic identification of Cinnamomum species by analyzing the nucleotide sequences of the partial non-coding internal transcribed space! 2 (ITS2) of ribosomal genes The genetic diversity of the five Cinnamomum species (Cinnamomum cassia. C zeylamcum, C louren C osmophloeum and C burmannu) was studied using polymerase chant reaction (PCR) amplification and a DNA sequencing method to molecularly identify the tested specimens Phylogenetic trees were constructed using several clustering methods, including unweighted pair-group method with arithmetic average (UPGMA), neighbor-joining. (NJ) and maximum evolution (ME) The eleven test samples were clustered into three major groups based on partial ITS2 sequences The phylogenetic results showed that C osmophloeum, and C burmann ale phylogenetically related to each other and that both ate inure closely related to C cassia Based on our results, there is enough sequence variation within the sequenced ITS2 regions to Identify individual specimens among the five tested Cinnamomum species Therefore, these ITS2 regions could be adopted as a molecular marker for differentiating among C cassia, C zeylamcum, C lour-cum, C osmophloem and C burmannu

• 出版日期2010-8