DEVELOPMENT AND CHARACTERIZATION OF MICROSATELLITE MARKERS FOR THE MEDICINAL PLANT SMILAX BRASILIENSIS (SMILACACEAE) AND RELATED SPECIES

作者：Martins Aline R^*; Abreu Aluana G; Bajay Miklos M; Villela Priscilla M S; Batista Carlos E A; Monteiro Mariza; Alves Pereira Alessandro; Figueira Glyn M; Pinheiro Jose B; Appezzato Da Gloria Beatriz; Zucchi Maria I

来源：Applications in Plant Sciences, 2013, 1(6): 1200507.

DOI：10.3732/apps.1200507

• medicinal plant
• microsatellites
• sarsaparilla
• Smilax
• transferability

Premise of the study: A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control.
Methods and Results: Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica.
Conclusions: The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.

• 出版日期2013-6