科研之友
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摘要
Introduction - The plant cuticle is a thin, predominantly lipid layer that covers all primary aerial surfaces of vascular plants. The monomeric building blocks of the cutin biopolymer are mainly omega-hydroxy fatty acids.
Objective - Analysis of omega-hydroxy fatty acids from cutin isolated from tomato fruits at different stages of decomposition in soil. Different derivatives and mass spectrometric techniques were used for peak identification and evaluation.
Methodology - Preparation of purified cutin involving dewaxing and HCl treatment. Incubation of purified cutin for 20 months in soil. Pentafluorobenzoyl derivatives were used for GC/MS operated in the electron capture negative ion (ECNI) mode and trimethylsilyl ethers for GC/MS operated in the electron ionisation (El) mode for analysis of omega-hydroxy fatty acids.
Results - Six omega-hydroxy fatty acids were detected in the purified cutin, three of which were identified as degradation products of 9,16-dihydroxyhexadecanoic acid as a consequence of the HCl treatment involved in the purification step. Incubation of the isolated cutin in soil was accompanied with decrease in concentration of all hydroxyl fatty acids.
Conclusion - We produced evidence that the HCl treatment only affected free hydroxyl groups and thus could be used for proportioning free and bound OH-groups on cutin fatty acids. The method enabled a direct quantification of the omega-hydroxy fatty acids throughout the incubation phase.
- 出版日期2010-12
