Down-regulation of WT1/+17AA gene expression using RNAi and modulating leukemia cell chemotherapy resistance

作者：Shen Huding; Xu Wenlin^*; Wu Zhaoyang; Tang Huarong; Xie Yanyan; Zhong Ximing

来源：Haematologica-The Hematology Journal, 2007, 92(9): 1270-1272.

DOI：10.3324/haematol.11010

• WT1
• isoform
• RNA interference
• multidrug resistance
• p-glycoprotein
• leukemia cell
• K562
• K562/A02

We have shown that inhibition of WT1/+17AA protein expression following transfection with a vector-based small interfering RNA expression construct in K562 cell lines, leads to a decrease in MDR1 and P-glycoprotein levels, accumulation of Rh123, and enhancement of the doxorubicin cytotoxicity. Our findings suggest that WT1/+17AA exerts its oncogenic function by modulating multidrug resistance in leukemia cells.

• 出版日期2007-9
• 单位江苏大学