Description of a Gram-negative bacterium, Sphingomonas guangdongensis sp nov.

作者：Feng, Guang-Da; Yang, Song-Zhen; Wang, Yong-Hong; Zhang, Xiu-Xiu; Zhao, Guo-Zhen; Deng, Ming-Rong; Zhu, Hong-Hui

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 5): 1697-1702.

DOI：10.1099/ijs.0.056853-0

• GENUS SPHINGOMONAS
• MAXIMUM-LIKELIHOOD
• SPHINGOPYXIS
• RECLASSIFICATION
• NOVOSPHINGOBIUM
• SPHINGOBIUM
• EMENDATION
• GENERA

A Gram-stain-negative bacterial strain, designated 9NM-8(T), was isolated from an abandoned lead-zinc ore in Mei county, Meizhou, Guangdong province, PR China. The isolate was orange-pigmented, aerobic, oxidase- and catalase-positive, motile with lophotrichous flagella and rod-shaped. Strain 9NM-8(T) grew optimally at pH 7.0 and 30 degrees C and in the absence of NaCl on R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 9NM-8(T) belongs to the genus Sphingomonas, with highest sequence similarities to Sphingomonas azotifigens KACC 14484(T) (96.1%), Sphingomonas trueperi DSM 7225(T) (96.0%) and Sphingomonas pituitosa DSM 13101(T) (95.6%). Strain 9NM-8(T) contained Q-10 as the predominant ubiquinone. The major fatty acids included C-18:1 omega 7c, C-16:0, C-16:1 omega 7c and/or C-16:1 omega 6C (summed feature 3) and 11-methyl C-18:1 omega 7c. The DNA G+C content was 69.6 +/- 1.3 mol%. The major component in the polyamine pattern was sym-homospermidine and the polar lipid profile contained sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and two unidentified phospholipids. Based on comparative analysis of physiological, chemotaxonomic and phylogenetic characteristics, strain 9NM-8(T) should be considered to represent a novel species of the genus Sphingomonas, for which the name Sphingomonas guangdongensis sp. nov. is proposed. The type strain is 9NM-8(T) (=GIMCC 1.653(T)=CGMCC 1.12672(T)=DSM 27570(T)).

• 出版日期2014-5
• 单位广东省微生物研究所